Serum Western Blot for the Detection of a c-Myc Protein Tag in Non-human Primates and Mice.

Blackwood, Meghan; Tang, Qiushi; Gruntman, Alisha M

Blackwood, Meghan; Tang, Qiushi; Gruntman, Alisha M.

Afiliação

Blackwood M; Department of Pediatrics and Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA.
Tang Q; Department of Pediatrics and Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA.
Gruntman AM; Department of Pediatrics and Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA. Alisha.Gruntman@UMassMed.edu.

Methods Mol Biol ; 2750: 107-112, 2024.

Article em En | MEDLINE | ID: mdl-38108971

ABSTRACT

ABSTRACT

This protocol allows for the detection of a c-Myc tag on alpha-1 antitrypsin (AAT) delivered to species that already have endogenous AAT such as non-human primates allowing reliable and repeatable semi-quantitation of serum levels of AAT.

Assuntos

Primatas; Animais; Camundongos; Western Blotting

Palavras-chave

AAT; Gene therapy; NHP; Protein levels; Serum levels

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Primatas Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google