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Comparison of the effect of Sodium Chloride concentration on protein determination: Bradford and Biuret methods.
Miranda, Matheus Pires.
Afiliação
  • Miranda MP; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, 81531-990, Curitiba, PR, Brazil. Electronic address: matheuspbioq@gmail.com.
Anal Biochem ; 687: 115450, 2024 04.
Article em En | MEDLINE | ID: mdl-38159714
ABSTRACT
Protein quantification methods using spectrophotometry are widely used in laboratory routines for different purposes. Samples generally contain non-protein components that can interfere with the quality of the analysis. A simple and quick test with different concentrations of sodium chloride demonstrated that the Bradford method is significantly affected by the presence of salt, while Biuret remains stable. Therefore, the choice of method is an important factor in reducing errors and ensuring more reliable results.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biureto Idioma: En Revista: Anal Biochem Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biureto Idioma: En Revista: Anal Biochem Ano de publicação: 2024 Tipo de documento: Article