Complex optically computed phase microscopy for label-free sub-cellular imaging.
Opt Contin
; 2(4): 793-800, 2023 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-38223255
ABSTRACT
In this study, we investigated a complex optically computed phase microscopy (complex-OCPM) technology. Based on a low coherence interferometer and an innovative optical computation approach, the complex-OCPM imaging system achieves depth resolved quantitative phase measurement. Particularly, the complex-OCPM imaging system directly measures the complex amplitude of the optical field emerging from the sample, extracts the phase as the argument of a complex signal, and achieves a high spatial resolution in phase imaging. We evaluated the performance of complex-OCPM imaging using resolution targets and live cells. Our results show that the complex-OCPM system achieves quantitative phase imaging with sub-cellular resolution on label-free cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Opt Contin
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos