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Single-cell transcriptomics reveals that glial cells integrate homeostatic and circadian processes to drive sleep-wake cycles.
Dopp, Joana; Ortega, Antonio; Davie, Kristofer; Poovathingal, Suresh; Baz, El-Sayed; Liu, Sha.
Afiliação
  • Dopp J; Center for Brain & Disease Research, VIB-KU Leuven, Leuven, Belgium.
  • Ortega A; Department of Neurosciences, KU Leuven, Leuven, Belgium.
  • Davie K; Leuven Brain Institute, KU Leuven, Leuven, Belgium.
  • Poovathingal S; Center for Brain & Disease Research, VIB-KU Leuven, Leuven, Belgium.
  • Baz ES; Department of Neurosciences, KU Leuven, Leuven, Belgium.
  • Liu S; Leuven Brain Institute, KU Leuven, Leuven, Belgium.
Nat Neurosci ; 27(2): 359-372, 2024 Feb.
Article em En | MEDLINE | ID: mdl-38263460
ABSTRACT
The sleep-wake cycle is determined by circadian and sleep homeostatic processes. However, the molecular impact of these processes and their interaction in different brain cell populations are unknown. To fill this gap, we profiled the single-cell transcriptome of adult Drosophila brains across the sleep-wake cycle and four circadian times. We show cell type-specific transcriptomic changes, with glia displaying the largest variation. Glia are also among the few cell types whose gene expression correlates with both sleep homeostat and circadian clock. The sleep-wake cycle and sleep drive level affect the expression of clock gene regulators in glia, and disrupting clock genes specifically in glia impairs homeostatic sleep rebound after sleep deprivation. These findings provide a comprehensive view of the effects of sleep homeostatic and circadian processes on distinct cell types in an entire animal brain and reveal glia as an interaction site of these two processes to determine sleep-wake dynamics.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sono / Ritmo Circadiano Limite: Animals Idioma: En Revista: Nat Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Bélgica

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sono / Ritmo Circadiano Limite: Animals Idioma: En Revista: Nat Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Bélgica