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The chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus uncovers patterns of genome evolution in mosquitoes.
Ryazansky, Sergei S; Chen, Chujia; Potters, Mark; Naumenko, Anastasia N; Lukyanchikova, Varvara; Masri, Reem A; Brusentsov, Ilya I; Karagodin, Dmitriy A; Yurchenko, Andrey A; Dos Anjos, Vitor L; Haba, Yuki; Rose, Noah H; Hoffman, Jinna; Guo, Rong; Menna, Theresa; Kelley, Melissa; Ferrill, Emily; Schultz, Karen E; Qi, Yumin; Sharma, Atashi; Deschamps, Stéphane; Llaca, Victor; Mao, Chunhong; Murphy, Terence D; Baricheva, Elina M; Emrich, Scott; Fritz, Megan L; Benoit, Joshua B; Sharakhov, Igor V; McBride, Carolyn S; Tu, Zhijian; Sharakhova, Maria V.
Afiliação
  • Ryazansky SS; Department of Entomology, Virginia Polytechnic and State University, Blacksburg, VA, USA.
  • Chen C; Department of Molecular Genetics of Cell, NRC "Kurchatov Institute", Moscow, Russia.
  • Potters M; Genetics, Bioinformatics, Computational Biology Program, Virginia Polytechnic and State University, Blacksburg, VA, USA.
  • Naumenko AN; Department of Biochemistry, Virginia Polytechnic and State University, Blacksburg, USA.
  • Lukyanchikova V; Department of Entomology, Virginia Polytechnic and State University, Blacksburg, VA, USA.
  • Masri RA; Department of Entomology, University of Maryland, College Park, MD, USA.
  • Brusentsov II; Department of Entomology, Virginia Polytechnic and State University, Blacksburg, VA, USA.
  • Karagodin DA; Group of Genomic Mechanisms of Development, Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Yurchenko AA; Laboratory of Structural and Functional Genomics, Novosibirsk State University, Novosibirsk, Russia.
  • Dos Anjos VL; Department of Entomology, Virginia Polytechnic and State University, Blacksburg, VA, USA.
  • Haba Y; Department of Entomology, Virginia Polytechnic and State University, Blacksburg, VA, USA.
  • Rose NH; Laboratory of Cell Differentiation Mechanisms, Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Hoffman J; Laboratory of Cell Differentiation Mechanisms, Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Guo R; Department of Entomology, Virginia Polytechnic and State University, Blacksburg, VA, USA.
  • Menna T; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA.
  • Kelley M; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA.
  • Ferrill E; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA.
  • Schultz KE; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, 20894, USA.
  • Qi Y; Department of Entomology, University of Maryland, College Park, MD, USA.
  • Sharma A; Department of Entomology, University of Maryland, College Park, MD, USA.
  • Deschamps S; Department of Biological Sciences, University of Cincinnati, Cincinnati, OH, USA.
  • Llaca V; County of San Diego Vector Control Program, San Diego, CA, USA.
  • Mao C; Mosquito and Vector Management District of Santa Barbara County, Santa Barbara, CA, USA.
  • Murphy TD; Department of Biochemistry, Virginia Polytechnic and State University, Blacksburg, USA.
  • Baricheva EM; Department of Biochemistry, Virginia Polytechnic and State University, Blacksburg, USA.
  • Emrich S; Corteva Agriscience, Johnston, IA, USA.
  • Fritz ML; Corteva Agriscience, Johnston, IA, USA.
  • Benoit JB; Biocomplexity Institute & Initiative University of Virginia, Charlottesville, VA, USA.
  • Sharakhov IV; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, 20894, USA.
  • McBride CS; Laboratory of Cell Differentiation Mechanisms, Institute of Cytology and Genetics, Novosibirsk, Russia.
  • Tu Z; Department of Electrical Engineering & Computer Science, the University of Tennessee, Knoxville, TN, USA.
  • Sharakhova MV; Department of Entomology, University of Maryland, College Park, MD, USA.
BMC Biol ; 22(1): 16, 2024 Jan 25.
Article em En | MEDLINE | ID: mdl-38273363
ABSTRACT

BACKGROUND:

Understanding genome organization and evolution is important for species involved in transmission of human diseases, such as mosquitoes. Anophelinae and Culicinae subfamilies of mosquitoes show striking differences in genome sizes, sex chromosome arrangements, behavior, and ability to transmit pathogens. However, the genomic basis of these differences is not fully understood.

METHODS:

In this study, we used a combination of advanced genome technologies such as Oxford Nanopore Technology sequencing, Hi-C scaffolding, Bionano, and cytogenetic mapping to develop an improved chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus.

RESULTS:

We then used this assembly to annotate odorant receptors, odorant binding proteins, and transposable elements. A genomic region containing male-specific sequences on chromosome 1 and a polymorphic inversion on chromosome 3 were identified in the Cx. quinquefasciatus genome. In addition, the genome of Cx. quinquefasciatus was compared with the genomes of other mosquitoes such as malaria vectors An. coluzzi and An. albimanus, and the vector of arboviruses Ae. aegypti. Our work confirms significant expansion of the two chemosensory gene families in Cx. quinquefasciatus, as well as a significant increase and relocation of the transposable elements in both Cx. quinquefasciatus and Ae. aegypti relative to the Anophelines. Phylogenetic analysis clarifies the divergence time between the mosquito species. Our study provides new insights into chromosomal evolution in mosquitoes and finds that the X chromosome of Anophelinae and the sex-determining chromosome 1 of Culicinae have a significantly higher rate of evolution than autosomes.

CONCLUSION:

The improved Cx. quinquefasciatus genome assembly uncovered new details of mosquito genome evolution and has the potential to speed up the development of novel vector control strategies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aedes / Culex Limite: Animals / Humans / Male Idioma: En Revista: BMC Biol Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aedes / Culex Limite: Animals / Humans / Male Idioma: En Revista: BMC Biol Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos