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Electrochemical detection of glutathione based on accelerated CRISPR/Cas12a trans-cleavage with MnO2 nanosheets.
Xia, Renpeng; Ouyang, Nan; Wang, Tingting; Zhuang, Yuan; Miao, Peng.
Afiliação
  • Xia R; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China. miaopeng@sibet.ac.cn.
  • Ouyang N; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China. miaopeng@sibet.ac.cn.
  • Wang T; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China. miaopeng@sibet.ac.cn.
  • Zhuang Y; Affiliated Hospital of Nantong University, Nantong 226001, China. 834634366@qq.com.
  • Miao P; Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China. miaopeng@sibet.ac.cn.
Chem Commun (Camb) ; 60(15): 2034-2037, 2024 Feb 15.
Article em En | MEDLINE | ID: mdl-38284796
ABSTRACT
The CRISPR/Cas12a system is accelerated by glutathione-mediated reduction of MnO2 nanosheets. By monitoring the trans-cleavage of the DNA probe, an electrochemical method for glutathione assay is fabricated, with the detection limit of 3.5 pM. It provides a promising tool for plasma analysis with satisfactory performance, indicating the broad application prospects of this glutathione assay.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Sistemas CRISPR-Cas Tipo de estudo: Diagnostic_studies Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Sistemas CRISPR-Cas Tipo de estudo: Diagnostic_studies Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China