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Rat hepatocytes secrete free oligosaccharides.
Huang, Chengcheng; Seino, Junichi; Honda, Akinobu; Fujihira, Haruhiko; Wu, Di; Okahara, Kyohei; Kitazume, Shinobu; Nakaya, Shuichi; Kitajima, Ken; Sato, Chihiro; Suzuki, Tadashi.
Afiliação
  • Huang C; Glycometabolic Biochemistry Laboratory, RIKEN-Cluster for Pioneering Research, Wako, Saitama, Japan.
  • Seino J; Glycometabolic Biochemistry Laboratory, RIKEN-Cluster for Pioneering Research, Wako, Saitama, Japan.
  • Honda A; Glycometabolic Biochemistry Laboratory, RIKEN-Cluster for Pioneering Research, Wako, Saitama, Japan.
  • Fujihira H; Glycometabolic Biochemistry Laboratory, RIKEN-Cluster for Pioneering Research, Wako, Saitama, Japan.
  • Wu D; Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Japan; Institute for Glyco-core Research (iGCORE), Nagoya University, Chikusa, Nagoya, Japan.
  • Okahara K; Discovery Concept Validation Function, KAN Research Institute, Inc, Kobe, Japan.
  • Kitazume S; Department of Clinical Laboratory Sciences, School of Health Sciences, Fukushima Medical University, Fukushima, Japan.
  • Nakaya S; Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan.
  • Kitajima K; Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Japan; Institute for Glyco-core Research (iGCORE), Nagoya University, Chikusa, Nagoya, Japan.
  • Sato C; Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya, Japan; Institute for Glyco-core Research (iGCORE), Nagoya University, Chikusa, Nagoya, Japan.
  • Suzuki T; Glycometabolic Biochemistry Laboratory, RIKEN-Cluster for Pioneering Research, Wako, Saitama, Japan. Electronic address: tsuzuki_gm@riken.jp.
J Biol Chem ; 300(3): 105712, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38309509
ABSTRACT
We recently established a method for the isolation of serum-free oligosaccharides, and characterized various features of their structures. However, the precise mechanism for how these glycans are formed still remains unclarified. To further investigate the mechanism responsible for these serum glycans, here, we utilized rat primary hepatocytes to examine whether they are able to secrete free glycans. Our findings indicated that a diverse array of free oligosaccharides such as sialyl/neutral free N-glycans (FNGs), as well as sialyl lactose/LacNAc-type glycans, were secreted into the culture medium by primary hepatocytes. The structural features of these free glycans in the medium were similar to those isolated from the sera of the same rat. Further evidence suggested that an oligosaccharyltransferase is involved in the release of the serum-free N-glycans. Our results indicate that the liver is indeed secreting various types of free glycans directly into the serum.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Hepatócitos Limite: Animals / Humans / Male Idioma: En Revista: J Biol Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Hepatócitos Limite: Animals / Humans / Male Idioma: En Revista: J Biol Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão