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New uses for an old technique: live imaging on the slice organ culture to study reproductive processes†.
Amato, Ciro Maurizio; Yao, Humphrey Hung-Chang.
Afiliação
  • Amato CM; Reproductive Developmental Biology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.
  • Yao HH; Reproductive Developmental Biology Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.
Biol Reprod ; 110(6): 1055-1064, 2024 Jun 12.
Article em En | MEDLINE | ID: mdl-38315794
ABSTRACT
Reproductive processes are dynamic and involve extensive morphological remodeling and cell-cell interactions. Live imaging of organs enhances our understanding of how biological processes occur in real time. Slice culture is a type of organ culture where thick slices are collected from an organ and cultured for several days. Slice culture is a useful and easy-to-implement technique for live imaging of reproductive events at cellular resolution. Here we describe a pipeline of live imaging on slice culture to visualize the process of urethra closure in mouse embryonic penis as a proof of principle. In combination with genetic reporter mice, nuclear stains, and exposure experiments, we demonstrate the feasibility of slice culture on a reproductive organ. We also provide a step-by-step protocol and troubleshooting guide to facilitate the adoption of slice culture with live imaging in other reproductive organs. Lastly, we discuss potential utilities and experiments that could be implemented with slice culture in reproductive sciences.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Órgãos Limite: Animals Idioma: En Revista: Biol Reprod Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Órgãos Limite: Animals Idioma: En Revista: Biol Reprod Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos