Transcriptome variation in banded newt (Ommatotriton vittatus) during its life cycle and habitat transition.
Comp Biochem Physiol Part D Genomics Proteomics
; 50: 101203, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38325219
ABSTRACT
Israel represents the southern limit of the distribution of the banded newt (Ommatotriton vittatus). The life cycle of O. vittatus includes several distinct phases eggs, aquatic larvae, a terrestrial phase and an aquatic reproductive phase. We investigated differences in gene expression during the life cycle and transition of banded newts between terrestrial and aquatic habitats using mRNA-seq. We identified â¼10 k genes that were differentially expressed (DE) in one of the pairwise comparisons between 3 groups 1 - terrestrial newts (males and females), 2 - aquatic newts (males and females), 3 - aquatic larvae before metamorphosis. The groups were clearly defined by Principal Components Analysis (PCA). The greatest difference was between aquatic newts (males and females) and aquatic larvae â¼7.4 k DE genes. Of special interest were the â¼2.4 k genes DE between the aquatic and terrestrial phenotypes. These included prominent candidates with known roles in kidney function (uromodulin homologs were strongly associated with aquatic lifestyle), tissue structure (keratins), and the thyroid hormone signaling modulator DUOXA1. Additional developmental and metabolic pathways overrepresented among the identified DE genes included "epidermis development", "nervous system development", "nucleotide-sugar biosynthesis". Overall, both metamorphosis and environmental transition of banded newts involve extensive transcriptomic remodeling involving developmental, metabolic, and cellular pathways. Understanding the roles of these pathways and individual genes is instrumental for studies of transition between habitats, especially those affected by climate change. Furthermore, the phenotypic flexibility of the newt and the underlying regulation of gene expression can shed light on the evolution of terrestrial vertebrates.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salamandridae
/
Ecossistema
/
Transcriptoma
/
Estágios do Ciclo de Vida
Limite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol Part D Genomics Proteomics
Assunto da revista:
BIOLOGIA
/
GENETICA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Israel