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Evolution of pH-sensitive transcription termination during adaptation to repeated long-term starvation.
Worthan, Sarah B; McCarthy, Robert D P; Delaleau, Mildred; Stikeleather, Ryan; Bratton, Benjamin P; Boudvillain, Marc; Behringer, Megan G.
Afiliação
  • Worthan SB; Department of Biological Sciences, Vanderbilt University, Nashville, TN.
  • McCarthy RDP; Evolutionary Studies Initiative, Vanderbilt University, Nashville, TN.
  • Delaleau M; Vanderbilt Institute for Infection, Immunology and Inflammation, Nashville, TN.
  • Stikeleather R; Department of Biological Sciences, Vanderbilt University, Nashville, TN.
  • Bratton BP; Centre de Biophysique Moléculaire, CNRS UPR4301, affiliated with Université d'Orléans, rue Charles Sadron, 45071 Orléans cedex 2, France.
  • Boudvillain M; Biodesign Center for Mechanisms of Evolution, Arizona State University, Tempe, AZ.
  • Behringer MG; Evolutionary Studies Initiative, Vanderbilt University, Nashville, TN.
bioRxiv ; 2024 Mar 01.
Article em En | MEDLINE | ID: mdl-38464051
ABSTRACT
Fluctuating environments that consist of regular cycles of co-occurring stress are a common challenge faced by cellular populations. For a population to thrive in constantly changing conditions, an ability to coordinate a rapid cellular response is essential. Here, we identify a mutation conferring an arginine-to-histidine (Arg to His) substitution in the transcription terminator Rho. The rho R109H mutation frequently arose in E. coli populations experimentally evolved under repeated long-term starvation conditions, during which feast and famine result in drastic environmental pH fluctuations. Metagenomic sequencing revealed that populations containing the rho mutation also possess putative loss-of-function mutations in ydcI, which encodes a recently characterized transcription factor associated with pH homeostasis. Genetic reconstructions of these mutations show that the rho allele confers a plastic alkaline-induced reduction of Rho function that, when found in tandem with a ΔydcI allele, leads to intracellular alkalinization and genetic assimilation of Rho mutant function. We further identify Arg to His substitutions at analogous sites in rho alleles from species originating from fluctuating alkaline environments. Our results suggest that Arg to His substitutions in global regulators of gene expression can serve to rapidly coordinate complex responses through pH sensing and shed light on how cellular populations across the tree of life use environmental cues to coordinate rapid responses to complex, fluctuating environments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article