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Single Cell Analysis of Proteoforms.
Su, Pei; Hollas, Michael A R; Butun, Fatma Ayaloglu; Kanchustambham, Vijaya Lakshmi; Rubakhin, Stanislav; Ramani, Namrata; Greer, Joseph B; Early, Bryan P; Fellers, Ryan T; Caldwell, Michael A; Sweedler, Jonathan V; Kafader, Jared O; Kelleher, Neil L.
Afiliação
  • Su P; Departments of Molecular Biosciences, Chemistry, Chemical and Biological Engineering, and Feinberg School of Medicine, Northwestern University, Evanston, Illinois 60208, United States.
  • Hollas MAR; Departments of Molecular Biosciences, Chemistry, Chemical and Biological Engineering, and Feinberg School of Medicine, Northwestern University, Evanston, Illinois 60208, United States.
  • Butun FA; Proteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois 60208, United States.
  • Kanchustambham VL; Proteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois 60208, United States.
  • Rubakhin S; Beckman Institute and Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
  • Ramani N; Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, United States.
  • Greer JB; Departments of Molecular Biosciences, Chemistry, Chemical and Biological Engineering, and Feinberg School of Medicine, Northwestern University, Evanston, Illinois 60208, United States.
  • Early BP; Departments of Molecular Biosciences, Chemistry, Chemical and Biological Engineering, and Feinberg School of Medicine, Northwestern University, Evanston, Illinois 60208, United States.
  • Fellers RT; Departments of Molecular Biosciences, Chemistry, Chemical and Biological Engineering, and Feinberg School of Medicine, Northwestern University, Evanston, Illinois 60208, United States.
  • Caldwell MA; Proteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois 60208, United States.
  • Sweedler JV; Beckman Institute and Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
  • Kafader JO; Departments of Molecular Biosciences, Chemistry, Chemical and Biological Engineering, and Feinberg School of Medicine, Northwestern University, Evanston, Illinois 60208, United States.
  • Kelleher NL; Proteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois 60208, United States.
J Proteome Res ; 23(6): 1883-1893, 2024 Jun 07.
Article em En | MEDLINE | ID: mdl-38497708
ABSTRACT
We introduce single cell Proteoform imaging Mass Spectrometry (scPiMS), which realizes the benefit of direct solvent extraction and MS detection of intact proteins from single cells dropcast onto glass slides. Sampling and detection of whole proteoforms by individual ion mass spectrometry enable a scalable approach to single cell proteomics. This new scPiMS platform addresses the throughput bottleneck in single cell proteomics and boosts the cell processing rate by several fold while accessing protein composition with higher coverage.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Proteômica / Análise de Célula Única Limite: Humans Idioma: En Revista: J Proteome Res / J. proteome res / Journal of proteome research Assunto da revista: BIOQUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Proteômica / Análise de Célula Única Limite: Humans Idioma: En Revista: J Proteome Res / J. proteome res / Journal of proteome research Assunto da revista: BIOQUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos