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Development of Transmission Ambient Pressure Laser Desorption Ionization/Postphotoionization Mass Spectrometry Imaging.
Qi, Keke; Lv, Yongmei; Xiong, Ying; Tian, Changlin; Liu, Chengyuan; Pan, Yang.
Afiliação
  • Qi K; National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, China.
  • Lv Y; High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
  • Xiong Y; Department of Dermatology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China.
  • Tian C; Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei 230026, China.
  • Liu C; High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
  • Pan Y; Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei 230026, China.
Anal Chem ; 96(14): 5489-5498, 2024 04 09.
Article em En | MEDLINE | ID: mdl-38527864
ABSTRACT
Laser-based high-resolution mass spectrometry imaging at ambient conditions has promising applications in life science. However, the ion yield during laser desorption/ablation is poor. Here, transmission atmospheric pressure laser desorption ionization combined with a compact postphotoionization (t-AP-LDI/PI) assembly with a krypton discharge lamp was developed for the untargeted imaging of various biomolecules. The spatial distributions of numerous lipid classes, fatty acids, neurotransmitters, and amino acids in the subregions of mouse cerebellum tissue were obtained. Compared with single laser ablation, the sensitivities for most analytes were increased by 1 to 3 orders of magnitude by dopant-assisted postphotoionization. After careful optimization, a spatial resolution of 4 µm could be achieved for the metabolites in mouse hippocampus tissue. Finally, the melanoma tissue slices were analyzed using t-AP-LDI/PI MSI, which revealed the metabolic heterogeneity of the melanoma microenvironment and exhibited the phenomenon of abnormal proliferation and invasion trends in tumor cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Melanoma Limite: Animals Idioma: En Revista: Anal Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Melanoma Limite: Animals Idioma: En Revista: Anal Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China