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DNA at the whim of the water: environmental DNA as a course-based undergraduate research experience.
Nolan, Kathleen P; Grunspan, Daniel Z; Myler, Erika; Brimble, Nava; Heyland, Andreas; Hanner, Robert H.
Afiliação
  • Nolan KP; Department of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada.
  • Grunspan DZ; Department of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada.
  • Myler E; Department of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada.
  • Brimble N; Department of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada.
  • Heyland A; Department of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada.
  • Hanner RH; Department of Integrative Biology, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada.
Genome ; 67(8): 256-266, 2024 Aug 01.
Article em En | MEDLINE | ID: mdl-38593476
ABSTRACT
Course-based undergraduate research experiences (CUREs) increase student access to high impact research experiences. CUREs engage students in the scientific process by learning how to pose scientific questions, develop hypotheses, and generate data to test them. Environmental DNA (eDNA) is a growing field of research that is gaining accessibility through decreasing laboratory costs, which can make a foundation for multiple, engaging CUREs. This manuscript describes three case studies that used eDNA in an upper year undergraduate course. The first focusses on a systematic literature review of eDNA metadata reporting. The second describes the biomonitoring of brook trout in southern Ontario using eDNA. The third involves eDNA metabarcoding for freshwater fish detection in southern Ontario. Undergraduates were involved in the development and execution of experiments, scientific communication, the peer review process, and fundraising. Through this manuscript, we show the novel application of eDNA CUREs and provide a roadmap for other instructors interested in implementing similar projects. Interviews with seven students from these courses indicate the benefits experienced from taking these courses. We argue that the use of eDNA in CUREs should be expanded in undergraduate biology programs due to the benefit to students and the increasing accessibility of this technology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estudantes / DNA Ambiental Limite: Animals / Humans País/Região como assunto: America do norte Idioma: En Revista: Genome Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Estudantes / DNA Ambiental Limite: Animals / Humans País/Região como assunto: America do norte Idioma: En Revista: Genome Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Canadá