Your browser doesn't support javascript.
loading
Imaging the intracellular refractive index distribution (IRID) for dynamic label-free living colon cancer cells via circularly depolarization decay model (CDDM).
Wang, Huijun; Zhang, Lu; Huang, Jie; Yang, Zewen; Fan, Chen; Yuan, Li; Zhao, Hong; Zhang, Zhenxi; Liu, Xiaolong.
Afiliação
  • Wang H; State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
  • Zhang L; State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
  • Huang J; School of Instrument Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
  • Yang Z; State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
  • Fan C; State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
  • Yuan L; State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
  • Zhao H; School of Instrument Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
  • Zhang Z; First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710049, China.
  • Liu X; State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
Biomed Opt Express ; 15(4): 2451-2465, 2024 Apr 01.
Article em En | MEDLINE | ID: mdl-38633098
ABSTRACT
Label-free detection of intracellular substances for living cancer cells remains a significant hurdle in cancer pathogenesis research. Although the sensitivity of light polarization to intracellular substances has been validated, current studies are predominantly focused on tissue lesions, thus label-free detection of substances within individual living cancer cells is still a challenge. The main difficulty is to find specific detection methods along with corresponding characteristic parameters. With refractive index as an endogenous marker of substances, this study proposes a detection method of intracellular refractive index distribution (IRID) for label-free living colon cancer (LoVo) cells. Utilizing the circular depolarization decay model (CDDM) to calculate the degree of circular polarization (DOCP) modulated by the cell allows for the derivation of the IRID on the focal plane. Experiments on LoVo cells demonstrated the refractive index of single cell can be accurately and precisely measured, with precision of 10-3 refractive index units (RIU). Additionally, chromatin content during the interphases (G1, S, G2) of cell cycle was recorded at 56.5%, 64.4%, and 71.5%, respectively. A significantly finer IRID can be obtained compared to the phase measurement method. This method is promising in providing a dynamic label-free intracellular substances detection method in cancer pathogenesis studies.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biomed Opt Express Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biomed Opt Express Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China