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The Plasmodiophora brassicae Golgi-localized UPF0016 protein PbGDT1 mediates calcium but not manganese transport in yeast and Nicotiana benthamiana.
Musharaf Hossain, Md; Alinapon, Cresilda Vergara; Todd, Christopher D; Wei, Yangdou; Bonham-Smith, Peta C.
Afiliação
  • Musharaf Hossain M; Department of Biology, University of Saskatchewan, Saskatoon S7N5E2, Saskatchewan, Canada.
  • Alinapon CV; Department of Biology, University of Saskatchewan, Saskatoon S7N5E2, Saskatchewan, Canada.
  • Todd CD; Department of Biology, University of Saskatchewan, Saskatoon S7N5E2, Saskatchewan, Canada.
  • Wei Y; Department of Biology, University of Saskatchewan, Saskatoon S7N5E2, Saskatchewan, Canada.
  • Bonham-Smith PC; Department of Biology, University of Saskatchewan, Saskatoon S7N5E2, Saskatchewan, Canada. Electronic address: peta.bonhams@usask.ca.
Fungal Genet Biol ; 172: 103896, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38663635
ABSTRACT
Manganese and calcium homeostasis and signalling, in eukaryotic organisms, are regulated through membrane located pumps, channels and exchangers, including the Mn2+/Ca2+ uncharacterized protein family 0016 (UPF0016). Here we show that Plasmodiophora brassicae PbGDT1 is a member of the UPF0016 and an ortholog of Saccharomyces cerevisiae Gdt1p (GCR Dependent Translation Factor 1) protein involved in manganese homeostasis as well as the calcium mediated stress response in yeast. PbGDT1 complemented the ScGdt1p and ScPMR1 (Ca2+ ATPase) double null mutant under elevated calcium stress but not under elevated manganese conditions. In both yeast and Nicotiana benthamiana, PbGDT1 localizes to the Golgi apparatus, with additional ER association in N. benthamiana. Expression of PbGDT1 in N. benthamiana, suppresses BAX-triggered cell death, further highlighting the importance of calcium homeostasis in maintaining cell physiology and integrity in a stress environment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Nicotiana / Cálcio / Complexo de Golgi / Manganês Idioma: En Revista: Fungal Genet Biol Assunto da revista: GENETICA / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Nicotiana / Cálcio / Complexo de Golgi / Manganês Idioma: En Revista: Fungal Genet Biol Assunto da revista: GENETICA / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Canadá