Deciphering the crop-soil-enzyme C:N:P stoichiometry nexus: A 5-year study on manure-induced changes in soil phosphorus transformation and release risk.

ABSTRACT

Carbonnitrogenphosphorus (CNP) stoichiometry plays a vital role in regulating P transformation in agriculture ecosystems. However, the impact of balanced CNP stoichiometry in paddy soil, particularly regarding relative soil P transformation, remains unknown. This study explores the response of CNP stoichiometry to manure substitution and its regulatory role in soil P transformation, along with the associated release risk to the environment. Based on a 5-year field study, our findings reveal that replacing 30 % of chemical P fertilizer with pig manure (equal total NPK amounts with chemical P fertilizer treatment, named CFM) increased soil total C without altering soil total P, resulting in an elevated soil CP ratio, despite the homeostasis of crop stoichiometry. This increase promoted microbial diversity and the accumulation of organic P in the soil. The Proteobacteria and Actinobacteria produced lower CPEEA metabolism together, and enhanced in vivo turnover of P. Additionally, by integrating high-resolution dialysis (HR-Peeper), diffusive gradients in thin films (DGT), DGT-induced fluxes in the soil (DIFS), and sediment P release risk index (SPRRI) models, we observed that, in addition to organic P, CFM simultaneously increased soil Al-P, thereby weakening the diffusion and resupply capacity of P from soil solids to the solution. Consequently, this decrease in P release risk to the environment was demonstrated. Overall, this study establishes a connection between crop-soil-enzyme CNP stoichiometry, soil microorganisms, and soil P biogeochemical processes. The study further evaluates the P release risk to the environment, providing a novel perspective on both the direct and indirect effects of manure substitution on soil P cycling.