Winter Hive Debris Analysis Is Significant for Assessing the Health Status of Honeybee Colonies (Apis mellifera).
Insects
; 15(5)2024 May 13.
Article
em En
| MEDLINE
| ID: mdl-38786906
ABSTRACT
Honeybee diseases are one of the most significant and most common causes of honeybee colonies' weakness and death. An early diagnosis of subclinical infections is necessary to implement precautionary and control measures. Sampling debris from hive bottom boards is simple, non-invasive, and cheap. In this study, we collected winter debris samples in apiaries located in the continental part of Croatia. We used molecular methods, PCR and qPCR, for the first time to analyze those samples. Laboratory results were compared with the health condition and strength of honeybee colonies at an apiary in spring. Our study successfully identified the presence and quantity of various pathogens, including the presence of Vairimorpha spp. (Nosema spp.), quintefied Paenibacillus larvae, Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Deformed Wing Virus (DWV), and Sacbrood Virus (SBV). However, our analysis did not detect Melissococcus plutonius, Crithidia mellificae, Lotmaria passim, and Aethina tumida. Samples of winter debris were also examined for the presence and quantification of the V. destructor mites, and their natural mite fall was observed in spring. Honeybee colonies were simultaneously infected by an average of four to six pathogens. Some observed honeybee colonies developed characteristic symptoms, while others did not survive the winter.
Acute Bee Paralysis Virus; Aethina tumida; Black Queen Cell Virus; Crithidia mellificae; Deformed Wing Virus and Sacbrood Virus; Lotmaria passim; Melissococcus plutonius; PCR/qPCR; Paenibacillus larvae; Vairimorpha spp. (Nosema spp.); Varroa destructor; honeybee colony (Apis mellifera); samples of winter hive debris
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Insects
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Croácia