Targeted i<sup>6</sup>A-RNA degradation through sequential Fluorination-Azidation and Click reaction with imidazole-based probes.

Guo, Yuyang; Chen, Xiaoqian; Gan, Youfang; Li, Yuanyuan; Wang, Rui

Targeted i⁶A-RNA degradation through sequential Fluorination-Azidation and Click reaction with imidazole-based probes.

Guo, Yuyang; Chen, Xiaoqian; Gan, Youfang; Li, Yuanyuan; Wang, Rui.

Afiliação

Guo Y; Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Chen X; Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Gan Y; Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Li Y; Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Wang R; Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, Guangdong 518063, C

Bioorg Med Chem Lett ; 108: 129815, 2024 Aug 01.

Article em En | MEDLINE | ID: mdl-38795737

ABSTRACT

ABSTRACT

We report the use of trimethylsilyl azide and Selectfluor to implement a standard protocol targeted at the prenylated nucleic acid known as i6A-RNA. After optimizing the conditions, we applied this method to regulate a wide range of i6A-RNA species using synthetic imidazole-based probes (I-IV). We observed that prenylated nucleic acid plays a crucial role in the cell hemostasis in A549 cell lines.

Assuntos

Azidas; Química Click; Halogenação; Imidazóis; Humanos; Imidazóis/química; Imidazóis/síntese química; Azidas/química; Células A549; RNA/química; RNA/metabolismo; Estrutura Molecular; Estabilidade de RNA/efeitos dos fármacos

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azidas / Halogenação / Química Click / Imidazóis Limite: Humans Idioma: En Revista: Bioorg Med Chem Lett Assunto da revista: BIOQUIMICA / QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

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