Flow cytometric analysis of monoclonal islet cell surface antibodies reacting with RINm5AH insulinoma cells.

Two monoclonal islet cell surface antibodies, isolated following immunization with human pancreatic islets, were analyzed for reactivity with the insulin-producing rat islet tumor RINm5AH cell line using computer assisted flow cytometric analysis. The antibody binding was quantified utilizing the gating facilities, single and dual parameter analysis of this system which are not available in conventional assays for cell surface antibody determination. The two antibodies specifically bind to the RINm5AH cells, although the dual parameter analysis demonstrated binding to only a subpopulation.