Niabella defluvii sp. nov., isolated from influent water of a wastewater treatment plant.
Int J Syst Evol Microbiol
; 74(6)2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38885037
ABSTRACT
Strain I65T (=KACC 22647T=JCM 35315T), a novel Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped, and orange-pigmented bacterium was isolated from influent water of a wastewater treatment system after treatment with several antibiotics, such as meropenem, gentamicin, and macrolide. The newly identified bacterial strain I65T exhibits significant multi-drug and heavy metal resistance characteristics. Strain I65T was grown in Reasoner's 2A medium [0â%-2â% (w/v) NaCl (optimum, 0â%), pH 5.0-10.0 (optimum, pH 7.0), and 20-45°C (optimum, 30â°C)]. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain I65T was closely related to Niabella yanshanensis CCBAU 05354T (99.56â% sequence similarity), Niabella hibiscisoli THG-DN5.5T (97.51â%), and Niabella ginsengisoli GR10-1T (97.09â%). Further analysis of the whole-genome sequence confirmed that the digital DNA-DNA hybridization, average nucleotide identity, and average amino acid identity values between strain I65T and N. yanshanensis CCBAU 05354T were 23.4, 80.7, and 85.0â%, respectively, suggesting that strain I65T is distinct from N. yanshanensis. The genome size of strain I65T was 6.1 Mbp, as assessed using the Oxford Nanopore platform, and its genomic DNA G+C content was 43.0âmol%. The major fatty acids of strain I65T were iso-C15ââ0 and iso-C15ââ1 G, and the major respiratory quinone was MK-7. Moreover, the major polar lipid of strain I65T was phosphatidylethanolamine. Based on genotypic, chemotaxonomic, and phenotype data, strain I65T represents a novel species belonging to the genus Niabella, for which the name Niabella defluvii sp. nov. is proposed. The type strain is I65T (=KACC 22647T=JCM 35315T).
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01-internacional
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MEDLINE
Assunto principal:
Filogenia
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Composição de Bases
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Análise de Sequência de DNA
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Ácidos Graxos
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Águas Residuárias
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Hibridização de Ácido Nucleico
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article