Assessing metabolic rates in zebrafish using a 3D-printed intermittent-flow respirometer and swim tunnel system.
Biol Open
; 13(6)2024 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-38887971
ABSTRACT
Zebrafish have become a widely used vertebrate model in physiology and reliable measures of their metabolic rate are needed. We have developed a 3D-printed respirometer and swim tunnel system and used it for obtaining accurate measurement of standard metabolic rate (SMR) and maximal, aerobic metabolic rate (MMR) in zebrafish under rest and maximal exercise, respectively. We compared a slow (stepwise) protocol to a fast (continuous) protocol for determining MMR. The fast protocol yielded slightly (but not significantly) higher oxygen consumption rates than the slow protocol and the data, in contrast to the slow protocol, followed a normal distribution. These findings point to the fast protocol as a fast and reliable method for obtaining accurate values of MMR in zebrafish. We make the 3D drawings for printing the system available to researchers, to help streamline the field of metabolic research in zebrafish and other smaller fish species.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Consumo de Oxigênio
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Natação
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Peixe-Zebra
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Impressão Tridimensional
Limite:
Animals
Idioma:
En
Revista:
Biol Open
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Dinamarca