Nocardioides agri sp. nov., isolated from garden soil.
Int J Syst Evol Microbiol
; 74(6)2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38888593
ABSTRACT
A novel bacterial strain, designated as MAH-18T, was isolated from soil sampled in a flower garden. Cells of strain MAH-18T were Gram-stain-positive, aerobic, motile, and rod-shaped. The colonies were beige in colour, smooth, and spherical when grown on Reasoner's 2A agar medium. Strain MAH-18T grew at 20-40â°C, pH 6.0-8.0, and 0-1.0â% NaCl. Cells were able to hydrolyse aesculin, gelatin, and Tween 20. According to the 16S rRNA gene sequence comparisons, the isolate was determined to be a member of the genus Nocardioides and most closely related to Nocardioides pyridinolyticus OS4T (97.9â%), Nocardioides hankookensis DS-30T (97.9â%), Nocardioides aquiterrae GW-9T (97.6â%), Nocardioides soli mbc-2T (97.5â%), Nocardioides conyzicola HWE 2-02T (97.4â%), and Nocardioides mangrovi GBK3QG-3T (96.3â%). Strain MAH-18T has a draft genome size of 4â788â325 bp (eight contigs), 4572 protein-coding genes, 46 tRNA, and three rRNA genes. The average nucleotide identity and digital DNA-DNA hybridization values between strain MAH-18T and the closest type strains were 81.5-83.4â% and 24.4-25.8â%, respectively. In silico genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAH-18T. The G+C content of the genomic DNA of strain was 72.2 mol% and the predominant isoprenoid quinone was MK-8 (H4). The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and unknown phospholipids. The major cellular fatty acids were determined to be C160 iso and C17ââ1 ω6c. The DNA-DNA hybridization results and phenotypic, genotypic, and chemotaxonomic data demonstrated that strain MAH-18T represents a novel species, for which the name Nocardioides agri sp. nov. is proposed, with MAH-18T as the type strain (=KACC 19744T=CGMCC 1.13656T).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Microbiologia do Solo
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Composição de Bases
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Análise de Sequência de DNA
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Ácidos Graxos
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Hibridização de Ácido Nucleico
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article