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Palladium/Platinum/Ruthenium Trimetallic Dendritic Nanozymes Exhibiting Enhanced Peroxidase-like Activity for Signal Amplification of Lateral Flow Immunoassays.
Wang, Weiguo; Cao, Qianqian; He, Jian; Xie, Yafeng; Zhang, Ying; Yang, Lin; Duan, Ming-Hui; Wang, Jikai; Li, Wei.
Afiliação
  • Wang W; Hengyang Medical School, Institute of Pharmacy and Pharmacology, The Affiliated Nanhua Hospital, Department of Hepatobiliary Surgery, University of South China, Hengyang 421000, Hunan, China.
  • Cao Q; Hengyang Medical School, Institute of Pharmacy and Pharmacology, The Affiliated Nanhua Hospital, Department of Hepatobiliary Surgery, University of South China, Hengyang 421000, Hunan, China.
  • He J; Hengyang Medical School, Institute of Pharmacy and Pharmacology, The Affiliated Nanhua Hospital, Department of Hepatobiliary Surgery, University of South China, Hengyang 421000, Hunan, China.
  • Xie Y; Department of Clinical Laboratory, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421000, Hunan, China.
  • Zhang Y; School of Biomedical and Chemical Engineering, Liaoning Institute of Science and Technology, Benxi 117004, China.
  • Yang L; Hengyang Medical School, Institute of Pharmacy and Pharmacology, The Affiliated Nanhua Hospital, Department of Hepatobiliary Surgery, University of South China, Hengyang 421000, Hunan, China.
  • Duan MH; Institute of Pathogenic Biology, Hengyang Medical School, University of South China, Hengyang 421000, Hunan, China.
  • Wang J; Hengyang Medical School, Institute of Pharmacy and Pharmacology, The Affiliated Nanhua Hospital, Department of Hepatobiliary Surgery, University of South China, Hengyang 421000, Hunan, China.
  • Li W; Hengyang Medical School, Institute of Pharmacy and Pharmacology, The Affiliated Nanhua Hospital, Department of Hepatobiliary Surgery, University of South China, Hengyang 421000, Hunan, China.
Nano Lett ; 24(27): 8311-8319, 2024 Jul 10.
Article em En | MEDLINE | ID: mdl-38935481
ABSTRACT
Developing ultrasensitive lateral flow immunoassays (LFIAs) has garnered significant attention in the field of point-of-care testing. In this study, a trimetallic dendritic nanozyme (Pd@Pt-Ru) was synthesized through Ru deposition on a Pd@Pt core and utilized to enhancing the sensitivity of LFIAs. Pd@Pt-Ru exhibited a Km value of 5.23 mM for detecting H2O2, which indicates an H2O2 affinity comparable with that of horseradish peroxidase. The Ru surface layer reduces the activation energy barrier, which increases the maximum reaction rate. As a proof of concept, the proposed Pd@Pt-Ru nanozyme was incorporated into LFIAs (A-Pd@Pt-Ru-LFIAs) for detecting human chorionic gonadotropin (hCG). Compared with conventional gold nanoparticle (AuNP)-LFIAs, A-Pd@Pt-Ru-LFIAs demonstrated 250-fold increased sensitivity, thereby enabling a visible detection limit as low as 0.1 IU/L. True positive and negative rates both reached 100%, which renders the proposed Pd@Pt-Ru nanozyme suitable for detecting hCG in clinical samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Paládio / Platina / Rutênio / Nanopartículas Metálicas / Limite de Detecção / Gonadotropina Coriônica / Peróxido de Hidrogênio Limite: Humans Idioma: En Revista: Nano Lett Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Paládio / Platina / Rutênio / Nanopartículas Metálicas / Limite de Detecção / Gonadotropina Coriônica / Peróxido de Hidrogênio Limite: Humans Idioma: En Revista: Nano Lett Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China