Hybrid nanoflower-based electrochemical lateral flow immunoassay for Escherichia coli O157 detection.
Mikrochim Acta
; 191(8): 453, 2024 07 06.
Article
em En
| MEDLINE
| ID: mdl-38970675
ABSTRACT
An electrochemical biosensor has been developed for detection of Escherichia coli O157 by integrating lateral flow with screen-printed electrodes. The screen-printed electrodes were attached under the lateral flow detection line, and organic-inorganic nanoflowers prepared from E. coli O157-specific antibodies as an organic component were attached to the lateral flow detection line. In the presence of E. coli O157, an organic-inorganic nanoflower-E. coli O157-antimicrobial peptide-labelled ferrocene sandwich structure is formed on the lateral flow detection line. Differential pulse voltammetry is applied using a smartphone-based device to monitor ferrocene on the detection line. The resulting electrochemical biosensor could specifically detect E. coli O157 with a limit of detection of 25 colony-forming units mL-1. Through substitution of antibodies of organic components in organic-inorganic nanoflowers, biosensors have great potential for the detection of other pathogens in biomedical research and clinical diagnosis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Escherichia coli O157
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Técnicas Eletroquímicas
Idioma:
En
Revista:
Mikrochim Acta
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China