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Impact of diet change on the gut microbiome of common marmosets (Callithrix jacchus).
Tang-Wing, Cassandra; Mohanty, Ipsita; Bryant, MacKenzie; Makowski, Katherine; Melendez, Daira; Dorrestein, Pieter C; Knight, Rob; Caraballo-Rodríguez, Andrés Mauricio; Allaband, Celeste; Jenné, Keith.
Afiliação
  • Tang-Wing C; Animal Care Program, University of California, San Diego, La Jolla, California, USA.
  • Mohanty I; Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California, USA.
  • Bryant M; Department of Pediatrics, University of California, San Diego, La Jolla, California, USA.
  • Makowski K; Animal Care Program, University of California, San Diego, La Jolla, California, USA.
  • Melendez D; Bioinformatics Graduate Program, University of California, San Diego, La Jolla, California, USA.
  • Dorrestein PC; Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California, USA.
  • Knight R; Center for Microbiome Innovation, University of California, San Diego, La Jolla, California, USA.
  • Caraballo-Rodríguez AM; Department of Pediatrics, University of California, San Diego, La Jolla, California, USA.
  • Allaband C; Center for Microbiome Innovation, University of California, San Diego, La Jolla, California, USA.
  • Jenné K; Department of Computer Science and Engineering, University of California, San Diego, La Jolla, California, USA.
mSystems ; 9(8): e0010824, 2024 Aug 20.
Article em En | MEDLINE | ID: mdl-38975760
ABSTRACT
Gastrointestinal diseases are the most frequently reported clinical problems in captive common marmosets (Callithrix jacchus), often affecting the health and welfare of the animal and ultimately their use as a research subject. The microbiome has been shown to be intimately connected to diet and gastrointestinal health. Here, we use shotgun metagenomics and untargeted metabolomics in fecal samples of common marmosets collected before, during, and after a dietary transition from a biscuit to a gel diet. The overall health of marmosets, measured as weight recovery and reproductive outcome, improved after the diet transition. Moreover, each marmoset pair had significant shifts in the microbiome and metabolome after the diet transition. In general, we saw a decrease in Escherichia coli and Prevotella species and an increase in Bifidobacterium species. Untargeted metabolic profiles indicated that polyamine levels, specifically cadaverine and putrescine, were high after diet transition, suggesting either an increase in excretion or a decrease in intestinal reabsorption at the intestinal level. In conclusion, our data suggest that Bifidobacterium species could potentially be useful as probiotic supplements to the laboratory marmoset diet. Future studies with a larger sample size will be beneficial to show that this is consistent with the diet change. IMPORTANCE Appropriate diet and health of the common marmoset in captivity are essential both for the welfare of the animal and to improve experimental outcomes. Our study shows that a gel diet compared to a biscuit diet improves the health of a marmoset colony, is linked to increases in Bifidobacterium species, and increases the removal of molecules associated with disease. The diet transition had an influence on the molecular changes at both the pair and time point group levels, but only at the pair level for the microbial changes. It appears to be more important which genes and functions present changed rather than specific microbes. Further studies are needed to identify specific components that should be considered when choosing an appropriate diet and additional supplementary foods, as well as to validate the benefits of providing probiotics. Probiotics containing Bifidobacterium species appear to be useful as probiotic supplements to the laboratory marmoset diet, but additional work is needed to validate these findings.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Callithrix / Dieta / Microbioma Gastrointestinal Limite: Animals Idioma: En Revista: MSystems Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Callithrix / Dieta / Microbioma Gastrointestinal Limite: Animals Idioma: En Revista: MSystems Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos