Automated Neutrophil Quantification and Histological Score Estimation in Ulcerative Colitis.

ABSTRACT

BACKGROUND: In the management of ulcerative colitis (UC), histological remission is increasingly recognized as the ultimate goal. The absence of neutrophil infiltration is crucial for assessing remission. This study aimed to develop an artificial intelligence (AI) system capable of accurately quantifying and localizing neutrophils in UC biopsy specimens to facilitate histological assessment. METHODS: Our AI system, which incorporates semantic segmentation and object detection models, was developed to identify neutrophils in hematoxylin and eosin-stained whole slide images. The system assessed the presence and location of neutrophils within either the epithelium or lamina propria and predicted components of the Nancy Histological Index and the PICaSSO Histologic Remission Index. We evaluated the system's performance against that of experienced pathologists and validated its ability to predict future clinical relapse risk in patients with clinically remitted UC. The primary outcome measure was the clinical relapse rate, defined as a partial Mayo score of ≥3. RESULTS: The model accurately identified neutrophils, achieving a performance of 0.77, 0.81, and 0.79 for precision, recall, and F-score, respectively. The system's histological score predictions showed a positive correlation with the pathologists' diagnoses (Spearman's ρ = 0.68-0.80; P < .05). Among patients who relapsed, the mean number of neutrophils in the rectum was higher than in those who did not relapse. Furthermore, the study highlighted that higher AI-based PICaSSO Histologic Remission Index and Nancy Histological Index scores were associated with hazard ratios increasing from 3.2 to 5.0 for evaluating the risk of UC relapse. CONCLUSIONS: The AI system's precise localization and quantification of neutrophils proved valuable for histological assessment and clinical prognosis stratification.