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High-Throughput Metabolomics using 96-plex Isotope Tagging.
Armbruster, Michael R; Grady, Scott F; Cho, Kevin; Patti, Gary J; Bythell, Benjamin J; Arnatt, Christopher K; Edwards, James L.
Afiliação
  • Armbruster MR; Department of Chemistry and Biochemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri 63103, United States.
  • Grady SF; Department of Chemistry and Biochemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri 63103, United States.
  • Cho K; Department of Chemistry, Washington University in St. Louis, 1 Brookings Dr Rm 102, St. Louis, Missouri 63110, United States.
  • Patti GJ; Department of Chemistry, Washington University in St. Louis, 1 Brookings Dr Rm 102, St. Louis, Missouri 63110, United States.
  • Bythell BJ; Department of Chemistry and Biochemistry, Ohio University, 307 Chemistry Building, Athens, Ohio 45701, United States.
  • Arnatt CK; Department of Chemistry and Biochemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri 63103, United States.
  • Edwards JL; Department of Chemistry and Biochemistry, Saint Louis University, 3501 Laclede Ave, St. Louis, Missouri 63103, United States.
Anal Chem ; 96(32): 12937-12942, 2024 Aug 13.
Article em En | MEDLINE | ID: mdl-39082755
ABSTRACT
Liquid chromatography-mass spectrometry (LC-MS) based metabolomics suffers from extended duty cycles and matrix-dependent quantitation. Chemical tags with 96 unique masses are reported, which alleviate the metabolomic workflow bottleneck and allow for absolute quantitation. A metabolic screen for carboxylic acids was performed on mammalian cells deprived of various nutrients and showed 24% RSD and analysis of 288 samples in 2 h.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metabolômica Limite: Humans Idioma: En Revista: Anal Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metabolômica Limite: Humans Idioma: En Revista: Anal Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos