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Manipulating host secreted protein gene expression: an indirect approach by HPV11/16 E6/E7 to suppress PBMC cytokine secretion.
Zhong, Mei-Zhen; Xu, Mei-Nian; Zheng, Si-Qi; Cheng, Shu-Qiong; Zeng, Kang; Huang, Xiao-Wen.
Afiliação
  • Zhong MZ; Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Xu MN; Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Zheng SQ; Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Cheng SQ; Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Zeng K; Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China. nfpfkzk@126.com.
  • Huang XW; Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, China. huangxw@smu.edu.cn.
Virol J ; 21(1): 172, 2024 Aug 02.
Article em En | MEDLINE | ID: mdl-39095779
ABSTRACT
Human papillomavirus (HPV) 11/16 E6/E7 proteins have been recognized to be pivotal in viral pathogenesis. This study sought to uncover the potential mechanisms of how HPV11/16 E6/E7-transfected keratinocytes inhibit cytokine secretion in peripheral blood mononuclear cells (PBMC). Upon co-culturing HPV11/16 E6/E7-transfected keratinocytes with PBMC in a non-contact manner, we observed a marked decrease in various cytokines secreted by PBMC. To determine if this suppression was mediated by specific common secreted factors, we conducted transcriptomic sequencing on these transfected cells. This analysis identified 53 common differentially secreted genes in all four HPV-transfected cells. Bioinformatics analysis demonstrated these genes were predominantly involved in immune regulation. Results from quantitative PCR (qPCR) and an extensive literature review suggested the downregulation of 12 genes (ACE2, BMP3, BPIFB1, CLU, CST6, CTF1, HMGB2, MMP12, PDGFA, RNASE7, SULF2, TGM2), and upregulation of 7 genes (CCL17, CCL22, FBLN1, PLAU, S100A7, S100A8, S100A9), may be crucial in modulating tumor immunity and combating pathogenic infections, with genes S100A8 and S100A9, and IL-17 signaling pathway being particularly noteworthy. Thus, HPV11/16 E6/E7 proteins may inhibit cytokine secretion of immune cells by altering the expression of host-secreted genes. Further exploration of these genes may yield new insights into the complex dynamics of HPV infection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Proteínas Oncogênicas Virais / Citocinas Limite: Humans Idioma: En Revista: Virol J Assunto da revista: VIROLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucócitos Mononucleares / Proteínas Oncogênicas Virais / Citocinas Limite: Humans Idioma: En Revista: Virol J Assunto da revista: VIROLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China