[Incorporation of the ampicillin resistance transposon into the Escherichia coli K-12 chromosome and into plasmids]. / Vstraivanie transpozona ustoichivosti k ampitsillinu v khromosomu Escherichia coli K-12 i plazmidy.
Genetika
; 15(2): 209-19, 1979.
Article
em Ru
| MEDLINE
| ID: mdl-391645
ABSTRACT
The mutant pEG1 of R-factor RP4 with temperature-sensitive defect in replication, carrying a transposable ampicillin resistance element Tn1 was used to define the frequency of insertion of this element into Escherichia coli K-12 chromosome and some other plasmids. Our results indicate that the frequency of colony forming by bacteria with pEG1-factor on ampicillin medium in non-permissive conditions corresponds to the frequency of Tn1 insertion into bacterial chromosome or some other plasmid (in case when the strains are carrying a second plasmid). The frequency of Tn1 insertion into the chromosome is about 4.10(-4). The defect in recA gene produce no serious change in the frequency of Tn1 insertion into the bacterial chromosome. The translocation of Tn1 element from pEG1-factor to R483, R6 and ColE1 plasmids occurs at 10 to 100-fold-higher frequency than from the plasmid to the chromosome. The insertion of Tn1 into the F'-factor KLF10 and R-factor R64-11 occurs at far lower frequency than that to plasmids R6, R483, or ColE1.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Elementos de DNA Transponíveis
/
Escherichia coli
/
Ampicilina
Idioma:
Ru
Revista:
Genetika
Ano de publicação:
1979
Tipo de documento:
Article