Cloning and expression profiling of peptide YY in the brain of common carp, Cyprinus carpio.
Gen Comp Endocrinol
; : 114600, 2024 Aug 27.
Article
em En
| MEDLINE
| ID: mdl-39209004
ABSTRACT
Peptide YY (PYY) is an anorectic brain-gut pancreatic peptide that helps in feeding regulation by reducing appetite and is well characterized in mammals. The role of PYY in relation to brain is least studied in mammals as well as in lower vertebrates including fish, however high expression was evident in male reproductive tissue. In this regard, this study attempts to evaluate the significance of PYY in the brain of common carp, Cyprinus carpio. As a first step, the cDNA of PYY from brain of adult male carp was subcloned. Following which expression analysis was performed using juvenile and adult fish. The differential distribution pattern in various regions of brain and ontogeny expression analysis indicate that PYY may involve in physiological processes related to brain-pituitary axis. In addition, a significant decrease in neuropeptides Y expression was observed upon PYY- endoribonuclease-prepared small interfering RNA transfection in brain cells, in vitro indicating their plausible PYY-NPY interaction.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Gen Comp Endocrinol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Índia