Measurement of xylanase activity with insoluble xylan substrate.
Biochem J
; 226(2): 617-20, 1985 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-3922355
ABSTRACT
Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Xilanos
/
Xilosidases
/
Glicosídeo Hidrolases
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1985
Tipo de documento:
Article