In vitro simulation of neural trauma by laser.
J Neuropathol Exp Neurol
; 44(3): 268-84, 1985 May.
Article
em En
| MEDLINE
| ID: mdl-3989583
ABSTRACT
A serious lack of knowledge about central nervous system trauma is encountered on the cellular level where the inability to create precise experimental lesions of known magnitude has limited our understanding of the reactions of single cells to injury. We used a laser cell surgery technique developed in this laboratory to manipulate neurons in a controlled environment, in order to observe pathologic reactions during and immediately after the injury. This technique is especially suited for axonal and dendritic amputations close to the perikaryon. The laser provided three different physical modes of injury to neurites direct vaporization of cytoplasm, pressure wave damage from external vaporization of substrate material, and photobiologically-induced localized cytoskeletal destruction leading to the slow pinching of processes followed by transection. Our data indicated a great similarity between laser impact damage and the cellular damage produced by physical trauma to the central nervous system.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças do Sistema Nervoso Central
/
Lasers
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
J Neuropathol Exp Neurol
Ano de publicação:
1985
Tipo de documento:
Article