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Determination of methionine sulfoxide in proteins: comparison of a gas-chromatographic and electrophoretic method.
J Biochem Biophys Methods ; 11(4-5): 241-9, 1985 Oct.
Article em En | MEDLINE | ID: mdl-4067174
ABSTRACT
Two methods for the determination of methionine in proteins have been used to estimate the extent of methionine sulfoxide obtained upon exposure of proteins to oxidizing agents. Both methods are based on prior treatment with cyanogen bromide, which attacks methionines (but not the sulfoxide derivative) with the resultant formation of methyl thiocyanate and peptides. The amount of methyl thiocyanate is determined quantitatively by gas chromatography, while the number of peptides is ascertained by SDS-polyacrylamide gel electrophoresis. The gas chromatographic estimate of CH3SCN offers an accurate and precise method (down to nanogram values) for the quantitative determination of methionine sulfoxide in proteins. Due to its simplicity and the use of low-cost equipment, the electrophoretic method appears to be a valuable complement to the gas chromatographic method, and the two methods in conjunction provide novel results.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos de Tosil / Proteínas / Metionina Idioma: En Revista: J Biochem Biophys Methods Ano de publicação: 1985 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos de Tosil / Proteínas / Metionina Idioma: En Revista: J Biochem Biophys Methods Ano de publicação: 1985 Tipo de documento: Article