Modifications of circular DNA by photoalkylation.
Radiat Res
; 103(1): 114-21, 1985 Jul.
Article
em En
| MEDLINE
| ID: mdl-4070556
ABSTRACT
The effects of photoalkylation on superhelical PM2 DNA were examined. The chief product was 8-(2-hydroxy-2-propyl)guanine, formed exclusively in sequences of alternating purines and pyrimidines. Other purine damages included 8-(2-hydroxy-2-propyl)adenine and smaller quantities of two uncharacterized adenine products. DNA strand breaks were formed with increasing irradiation. A small quantity of thymine-containing photodimers was formed. Photoalkylation of poly(dG-dC)poly(dG-dC) reduced the concentration of salt required to effect inversion of the circular dichroic spectrum. This suggests that photoalkylation induces the transition of poly(dG-dC)poly(dG-dC) from the right-handed B form of DNA to the left-handed Z form.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Circular
/
DNA Viral
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Radiat Res
Ano de publicação:
1985
Tipo de documento:
Article