Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017.
Gene
; 31(1-3): 225-31, 1984 Nov.
Article
em En
| MEDLINE
| ID: mdl-6098527
ABSTRACT
We have cloned the DNA encoding the haemolysin of Vibrio cholerae El Tor strain 017 into the plasmid vector pBR322. The resultant plasmid, pPM431, has a 6.2-kb PstI DNA insert which leads to the production of the haemolysin in Escherichia coli K-12. Deletion analysis and transposon mutagenesis have allowed us to localize several regions affecting haemolysin production. A number of these mutants have been analysed in E. coli K-12 minicells. Three proteins have been identified A, 80 kDal; B, 71 kDal; and C, 22 kDal. A is the haemolysin which appears to be cell-associated in E. coli K-12, and B and C are required for its efficient production. We suggest that the genes for proteins A, B and C be designated hlyA, hlyB and hlyC, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vibrio cholerae
/
Genes Bacterianos
/
Proteínas Hemolisinas
Idioma:
En
Revista:
Gene
Ano de publicação:
1984
Tipo de documento:
Article