The structure of the covalent linkage between proteins and RNA in encephalomyocarditis virus.
Nucleic Acids Res
; 8(16): 3729-42, 1980 Aug 25.
Article
em En
| MEDLINE
| ID: mdl-6253907
ABSTRACT
Two protein, VPgA and VPgB, are covalently bound to the virion RNA of encephalomyocarditis (EMC) virus. Their molecular weightrs, as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate are 10,000 and 8,000, respectively. A study of nucleotide-peptides isolated from VPg-RNA compound has shown that VPgA is bound to the 5'-terminal nucleotide of RNA by a phosphodiester bond. The 5'-terminal nucleotide of RNA is uridylic acid. It is the hydroxy group of the Tyr residue of VPgA that is involved in the formation of the linkage with RNA. VPgB-RNA seems to be similar to VPgA-RNA both in the structure of the RNA-protein linkage and localization of VPgB on RNA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
RNA Viral
/
Vírus da Encefalomiocardite
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1980
Tipo de documento:
Article