Cell type-specificity of epidermal growth factor (EGF) binding in primary cultures of early postnatal mouse cerebellum.
Neurosci Lett
; 30(2): 179-82, 1982 May 28.
Article
em En
| MEDLINE
| ID: mdl-6287367
ABSTRACT
Binding of 125I-labeled epidermal growth factor (EGF) to cells of cultured early postnatal mouse cerebellar cells was investigated by autoradiography in conjunction with cell type-specific immunolabeling of neurons, astrocytes and oligodendrocytes. By use of tetanus toxin for recognition of neurons and glial fibrillary acidic (GFA) protein and 04 antigen as markers for astrocytes and oligodendrocytes, respectively, it could be shown that oligodendrocytes do not express receptors for EGF within the limits of sensitivity of the autoradiographic method, and that less than 1% of all small neurons (mostly granule cells) and 50-90% of all GFA protein-sensitive astrocytes show detectable levels of EGF binding.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cerebelo
/
Fator de Crescimento Epidérmico
Limite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
1982
Tipo de documento:
Article