Acid treatment enhances IL-2 activity of conditioned medium.
J Immunol Methods
; 67(1): 73-8, 1984 Feb 24.
Article
em En
| MEDLINE
| ID: mdl-6366068
ABSTRACT
Although conditioned medium (CM) from human lymphocytes or mononuclear cells is the most readily available source of interleukin-2 (IL-2) for human T cell culture, its IL-2 activity in our experience is inconsistent. It is likely that this is, at least in part, due to the presence of toxic substances in the CM. Using CM from TPA/SEA induced human mononuclear cells, we have found that acid treatment (pH 2.0, greater than 30 min) significantly improves its ability to promote T cell growth. It is postulated that the selection process which occurs in cultures of mitogen stimulated T cells may result in cells which are sensitive to mitogen-induced lymphotoxins and that these are inactivated by the acid treatment. Since acid treatment did not similarly improve the T cell growth promoting ability of PHA induced, lectin-free commercial IL-2, there must be other differences between it and our CM, which play a role in T cell growth.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação Linfocitária
/
Linfócitos T
/
Técnicas Imunológicas
/
Interleucina-2
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1984
Tipo de documento:
Article