Simple estimation of carrier binding capacity using sorption kinetics curve-fitting.

ABSTRACT

Kinetic curves of the sorption of biological-like compounds and proteins onto insoluble carriers were determined either by linearisation via double reciprocal transformation or by non-linear fitting using the following equation B = (1/K1) X [t/(t + K2/K1)]. Both these procedures provided non-significant differences in the values of parameters of sorption kinetics, namely of equilibrium sorption (BM), sorption half-time (t1/2) and initial sorption rate (vo). Moreover, these methods proved to be sufficient to provide a precise description of the kinetics of different types of sorption, such as chemical, physical, ionic, biospecific and non-specific sorption. Both computing procedures make it possible (i) to calculate the parameters BM, t1/2 and vo even in instances where they cannot be established experimentally, and (ii) to replace the graphic estimation with computation. The assumption that all types of sorption mentioned above will be kinetically controlled in a uniform way proved to be reasonable.