Specificity of the suppressive action of glucocorticoids on the proliferation of monocyte/macrophages in the CSF-stimulated cultures of mouse bone marrow.
Exp Hematol
; 11(3): 178-86, 1983 Mar.
Article
em En
| MEDLINE
| ID: mdl-6601026
ABSTRACT
Addition of cortisol and its analogs to soft-agar culture of bone marrow cells markedly decreased the number of monocyte colonies in the presence of colony-stimulating factor. Steroids of other categories and cortisol metabolites were much less inhibitory than cortisol, and the shape of dose-response curve apparently differed between cortisol and other steroids. The action of cortisol was not influenced by excess progesterone or testosterone. Addition of cortisol succinate in liquid cultures of CSF-stimulated cells caused a gradual decrease of cellular uptake of [3H]thymidine. The steroid also suppressed CSF-independent [3H]thymidine uptake, but the time course of suppression obviously differed from that of CSF-dependent uptake. On day 7 of incubation, the steroid-treated cultures contained a smaller number of cells but higher activities of lysosomal enzymes than the control cultures. These results show that the glucocorticoids inhibit proliferation of the cells of monocyte/macrophage lineage.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores Estimuladores de Colônias
/
Glucocorticoides
Limite:
Animals
Idioma:
En
Revista:
Exp Hematol
Ano de publicação:
1983
Tipo de documento:
Article