Immunofluorescence: a sensitive and rapid method for the detection of purine nucleoside phosphorylase in single cells.
J Immunol Methods
; 34(2): 127-32, 1980.
Article
em En
| MEDLINE
| ID: mdl-6770005
ABSTRACT
Indirect immunofluorescence was used as a rapid, sensitive and specific method for the visualization of the enzyme purine nucleoside phosphorylase in single cells. The enzyme was localized throughout cytoplasm of human lymphoblasts and fibroblasts but not in cell nuclei. This method is valuable for the detection of mutant enzyme protein in cell-mediated immunodeficiencies caused by purine nucleoside phosphorylase deficiency since it does not rely on enzyme activity. It requires only a limited number of cells and can therefore be used for the rapid screening for the presence of cross-reactive protein in immunodeficiency diseases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pentosiltransferases
/
Linfócitos
/
Imunofluorescência
/
Purina-Núcleosídeo Fosforilase
/
Fibroblastos
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1980
Tipo de documento:
Article