Use of an enzyme-linked immunosorbent assay (ELISA) for screening of hybridoma antibodies against cell surface antigens.
J Immunol Methods
; 39(4): 407-11, 1980.
Article
em En
| MEDLINE
| ID: mdl-6780627
ABSTRACT
A micro-ELISA for screening of antibodies from hybridoma cultures against surface antigens of human melanoma is described. The technique employs alkaline phosphatase-conjugated protein A and target cells attached to poly-L-lysine-coated microtiter plates. The micro-ELISA is equally sensitive as the radioimmunoassay. Mild glutaraldehyde treatment of cells did not lead to an appreciable loss of antigen activity. The fixed cells can be stored at 4 degrees C for at least 6 weeks. It is concluded that the ELISA is superior to the radioimmunoassay in the following aspects (1) exclusion of radioactive hazards, (2) speed of performance, and (3) lower costs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Híbridas
/
Anticorpos
/
Antígenos de Superfície
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1980
Tipo de documento:
Article