[Critical aspects of actual methods for high-density-lipoprotein cholesterol quantitation]. / Aspects critiques des méthodes actuelles de détermination du cholestérol-HDL.
Pathol Biol (Paris)
; 28(9): 617-20, 1980 Nov.
Article
em Fr
| MEDLINE
| ID: mdl-7003509
ABSTRACT
In this study we compare various methods for h;igh-density-lipoprotein cholesterol quantitation ultracentrifugation; electrophoresis either on cellulose acetate or on agarose gel; precipitation methods with polyanions-bivalent cations; adsorption procedure using concanavalin A (Con A). quantification by electrophoresis is slow and not precise. Except heparin-Mn++ and dextran (MM 5 X 10(5) and 2 X 10(6)) - CaCl2 procedures, precipitation and Con A methods own a good repeatability (VC 2%) and give a linear response after VLDL or LDL-VLDL addition to tested sera. Regression analysis of the data obtained using these methods in pairs demonstrate a correct accuracy (r > 0.95). The Student t-test shows that the usual values must be evaluated for each method. We suggest to use either phosphotungstate - Mg++ or Con A procedure as method for HDL cholesterol quantitation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colesterol
/
Lipoproteínas HDL
Idioma:
Fr
Revista:
Pathol Biol (Paris)
Ano de publicação:
1980
Tipo de documento:
Article