Interaction of zinc ions with DNA-dependent RNA polymerases A, B and C isolated from calf thymus.
Mol Biol Rep
; 8(2): 77-83, 1982 Mar 31.
Article
em En
| MEDLINE
| ID: mdl-7078552
ABSTRACT
Purified DNA-dependent RNA polymerases A, B and C isolated from calf thymus contain a significant amount of zinc. Atomic absorption spectroscopy revealed the presence of 6.7, 5.35 and 2.6--4.1 g-atoms of zinc per mole of polymerase A, B and C, respectively. These enzymes are inhibited by treatment with 1,10-phenanthroline at concentrations varying from 10(-5) to 10(-4) M. However, the addition of zinc ions does not restore fully the activity of 1,10-phenanthroline treated enzymes. Exogenous zinc ions reduce in vitro an overall RNA synthesis catalysed by RNA polymerases from calf thymus. In addition to the sites which bind zinc in a specific and stoichiometric way these enzymes possess other classes of binding sites with high and low affinity. Occupancy by exogenous zinc of these additional binding sites inhibit polymerase activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timo
/
Zinco
/
RNA Polimerases Dirigidas por DNA
Limite:
Animals
Idioma:
En
Revista:
Mol Biol Rep
Ano de publicação:
1982
Tipo de documento:
Article