Erythromycin binding to human serum.
Biochem Pharmacol
; 31(6): 1081-7, 1982 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-7082362
ABSTRACT
Erythromycin binding to human serum was measured under conditions of binding equilibrium. The binding is sensitive to pH changes, decreasing at acid pH. Over a great range of serum dilution, the bound fraction is semilogarithmically related to serum concentration. Binding is shown to be completely reversible. With increasing erythromycin concentration a specific part of binding is saturable and specifically displaceable by erythromycin is specifically bound to a single class of noninteracting binding sites with an apparent dissociation constant Kd = 5.9 microM (38 degrees C). The kinetic and thermodynamic parameters at 25 degrees are Kd = 8.4 microM, delta H degrees = +4.4 X 10(3) cal per mole, delta G degrees = 6.9 X 10(3) cal per mole, delta S degrees = +38 e.u.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Sanguíneas
/
Eritromicina
Limite:
Humans
Idioma:
En
Revista:
Biochem Pharmacol
Ano de publicação:
1982
Tipo de documento:
Article