A differential labelling model for determining the number of catalytically essential carboxyl groups in fumarase.
Biochim Biophys Acta
; 662(2): 196-201, 1981 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-7317435
ABSTRACT
We have developed a mathematical model of the nonideal case in which enzymatic activity changes may also result from modification of non-essential groups. As an illustration of this method, the number of essential carboxyl groups in pig heart fumarase (L-malate hydro-lyase, ED 4.2.1.2) was determined by the differential labeling technique. Enzymatic activity was related to the number of modified carboxyl groups according to the model and the results were compatible with the existence of two essential carboxyl groups in fumarase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fumarato Hidratase
/
Fumaratos
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1981
Tipo de documento:
Article