Interactions of atrial natriuretic peptide with A7r5 cells.
Biochem Biophys Res Commun
; 204(3): 1286-91, 1994 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-7526862
ABSTRACT
The interactions of Atrial Natriuretic Peptide with its receptors were investigated with the vascular smooth muscle cells from the A7r5 cell line. Displacement experiments of [125I]rat-Atrial Natriuretic Peptide by rat-Atrial Natriuretic Peptide revealed a single class of receptors with a Kd = 0.21 +/- 0.08 nM and a Bmax = of 35 +/- 16 fmol/mg of protein. Furthermore, the complex [125I]r-Atrial Natriuretic Peptide was internalised by a heatsensitive process. Finally, Atrial Natriuretic Peptide increased cyclic Guanosyl 3',5' Mono Phosphate in a time dependent and dose dependent way, a concentration of 0.1 microM increasing cyclic Guanosyl 3',5' Mono Phosphate level by a factor of 8.3 times when compared to basal level.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator Natriurético Atrial
/
Receptores do Fator Natriurético Atrial
/
Músculo Liso Vascular
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Reino Unido