Effects of dopamine on adenylyl cyclase activity and amylase secretion in rat parotid tissue.
J Dent Res
; 74(6): 1289-94, 1995 Jun.
Article
em En
| MEDLINE
| ID: mdl-7543123
ABSTRACT
Several previous studies have shown that dopamine causes amylase secretion from rat parotid tissue. However, the mechanism of this dopamine action is still unclear. The present study was designed to characterize dopamine action in rat parotid gland tissue by examining the effects of dopamine on cyclic AMP accumulation, adenylyl cyclase activity, and amylase release. Dopamine significantly enhanced accumulation of cyclic AMP in parotid slices and stimulated adenylyl cyclase activity in parotid membrane preparations. It also significantly stimulated amylase release from parotid slices. The stimulatory effects of dopamine on cyclic AMP accumulation, adenylyl cyclase activity, and amylase release were effectively blocked with propranolol, a beta-adrenergic antagonist, but not by either SCH 23390, a preferential D1 antagonist, or butaclamol, a preferential D2 antagonist. No substantial specific binding sites for D1 receptors were detectable by [3H]SCH 23390 binding in parotid membranes. These results suggest that the stimulatory effect of dopamine on amylase secretion in rat parotid tissue is not mediated through specific D1 dopamine receptors but rather through beta-adrenergic receptors.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glândula Parótida
/
Dopamina
Limite:
Animals
Idioma:
En
Revista:
J Dent Res
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Japão