A member of the CTF/NF-1 transcription factor family regulates murine growth hormone receptor gene promoter activity.
Endocrinology
; 136(11): 5236-9, 1995 Nov.
Article
em En
| MEDLINE
| ID: mdl-7588264
ABSTRACT
Deletional analysis by transient transfection of COS-7 cells with the murine growth hormone (GH) receptor gene promoter for the L1 transcript identified a 16-bp enhancer element located approximately 3.0 kb upstream of the major transcription start sites. The sequence of this enhancer element suggested a binding motif for the CTF/NF-1 family of transcription factors. In electromobility shift assays (EMSA) this DNA-element formed a sequence-specific complex with nuclear proteins from COS-7 cells. Competition experiments with oligonucleotide containing a consensus binding motif for CTF/NF-1 protein(s) and supershift EMSA with an anti-CTF/NF-1 antibody established that a CTF/NF-1 like protein binds to this enhancer element.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Receptores da Somatotropina
/
Regulação da Expressão Gênica
/
Regiões Promotoras Genéticas
/
Proteínas Estimuladoras de Ligação a CCAAT
/
Proteínas de Ligação a DNA
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Endocrinology
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos