Production of hematopoietic growth factors by human B lymphocytes: mechanisms and possible implications.
Stem Cells
; 11 Suppl 2: 150-5, 1993 Jul.
Article
em En
| MEDLINE
| ID: mdl-7691321
ABSTRACT
In this study we have investigated the ability of human B lymphocytes to produce granulocyte-macrophage colony stimulating factor (GM-CSF) and, in preliminary experiments, granulocyte CSF (G-CSF). The sources of human B cells were surgically removed tonsils from normal individuals and peripheral blood from patients with B cell chronic lymphocytic leukemia (B-CLL). Tonsil B lymphocytes were purified by E rosetting and complement-mediated cytotoxicity with selected monoclonal antibodies and subsequently fractionated by a Percoll density gradient into in vivo activated and resting cells. The latter cell fractions were subsequently cultured with or without stimuli. GM-CSF was detected by a bioassay, G-CSF by an enzyme-linked immunoassay. In vivo and in vitro activated B cells produced GM-CSF, whereas in vivo activated, but not in vitro activated, B lymphocytes produced G-CSF. These results were confirmed by Northern blot experiments with cDNA probes specific for GM-CSF and G-CSF genes. Many B cell suspensions from B-CLL patients produced GM-CSF or G-CSF only following Staphylococcus Aureus Cowan I (SAC) stimulation; in some cases, a spontaneous production or no production at all of the two cytokines was detected. The possible implications of these results for B cell physiology and for the pathogenesis of immunologically mediated diseases will be discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Leucemia Linfocítica Crônica de Células B
/
Fator Estimulador de Colônias de Granulócitos
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
Limite:
Humans
Idioma:
En
Revista:
Stem Cells
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Itália